Guide Library Design: Researchers design a pooled CRISPR guide RNA library targeting genes of interest. Cell Transduction: The guide library is introduced into cells, typically via lentiviral ...

The team suggests TIGER tech could pave the way to the development of precise gene controls for use in CRISPR-based therapies.

Artificial intelligence can predict on- and off-target activity of CRISPR tools that target RNA instead of DNA, according to new research published in Nature Biotechnology.

"We anticipate that RNA-targeting Cas13 enzymes will have a large impact on molecular biology and medical applications, yet little is known about guide RNA design for high targeting efficacy," said ...

AI and CRISPR precisely control gene expression RNA-based predictive models developed Date: July 3, 2023 Source: Columbia University School of Engineering and Applied Science Summary: The study ...

A new review highlights the potential of CRISPR–Cas13 for RNA-editing therapies. Discussing the advantages of the effector protein Cas13 over Cas9, they report that CRISPR–Cas13 ...

Such information, the authors of the Molecular Cell article suggest, may open new design and engineering avenues for CRISPR technologies, setting the stage for more precise genetic modifications.

As CRISPR gene editing marches closer to the clinic, several researchers have been tinkering to unleash the DNA editor on another nucleic acid: RNA, the intermediary messenger between DNA and ...

Researchers have developed a new kind of CRISPR screen technology to target RNA. The team leveraged their technology for a critical analysis: The COVID-19 public health emergency is due to a ...