ChIP-seq, short for chromatin immunoprecipitation sequencing, combines chromatin immunoprecipitation with real-time next generation sequencing to identify genomic binding sites that proteins such ...

ChIP–seq experiments generate large quantities of data, and effective computational analysis will be crucial for uncovering biological mechanisms.

ChIP-seq data analysis workflow. ChIP DNA sequencing fastq files alignment (Bowtie) reference genome index peak calling (MACS/SICER) Gene lists (NCBI) in gene, downstream, upstream, enhancer sites.

In it, the scientists describe a user-friendly workflow for transforming raw sequencing files into normalized data for quantitative downstream analysis.

Avoiding the formation of unwanted clusters of similar elements when dividing data into groups is of great importance for the ...