This report describes a simple and rapid DNA methylation quantification method, which consists of treating genomic DNA extracted from a sample with bisulfite, amplifying a target gene by PCR ...

We have demonstrated the concordance between Meth-DOP-PCR and MSP and shown the application of this technique for the methylation analysis of DNA extracted from the serum of lung cancer patients.

DNA Methylation Detection Using Droplet Digital" PCR Hamutal Bonen, PhD; Jeremiah McDole, PhD; Eddy van Collenburg Bio-Rad Laboratories, Inc., Digital Biology Center, 5731 W Las Positas Blvd, ...

The laboratory is involved in the development and application of technologies to analyze DNA methylation, miRNAs and other epigenetic modifications quantitatively at high resolution at target loci and ...

Firstly, the methylation signal can be amplified and therefore DNA methylation analysis by PCR is very sensitive, although vigorous validation is needed to minimize false-positives.

Methylation is one of the most classical epigenetic modifications in eukaryotes. DNA methylation regulates gene expression and has important implications in both growth and disease-related research.

This technique allows scientists to see the results almost immediately while the process is still ongoing, whereas conventional RT–PCR only provides results at the end of the process. Real time RT–PCR ...