DNA extraction from plant tissues, such as leaves, requires the breakdown of the rigid cell wall either by chemical, enzymatic, mechanical means, or some combination of these three methods.

Before that DNA can be identified, though, it first has to be isolated from the sample material, typically via a multi-step process known as CTAB (cetyltrimethylammonium bromide) extraction.

DNA extraction kits are designed to be broad spectrum to work for both microbes and their host. In most plant-microbiome projects there will be a minute quantity of microbial DNA compared to the host.

Full extraction: Scientists elaborated a new method to extract DNA from viruses, bacteria, plants and animals Date: May 20, 2016 Source: Lomonosov Moscow State University Summary: A simple and ...